Recombinant Human IL-11 Protein (RP00050)

Active Recombinant Human IL-11 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 21 kDa.

Recombinant human IL11 promotes the proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.3-5ng/mL, corresponding to a specific activity of 2×10⁵-7.69×10⁵units/mg.

All(2)|

货号: RP00050

详细信息

种属

Human

表达宿主

E. coli

Calculated MW

19.14 kDa

Observed MW

21 kDa

标签

No tag

纯度

> 97% by SDS-PAGE.

内毒素

< 1.0 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation.

描述

Recombinant Human IL-11 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Pro22-Leu199) of human IL-11 (Accession #NP_000632.1) fused with an initial Met at the N-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.3-5 ng/mL, corresponding to a specific activity of 2×10⁵-7.69×10⁵units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (1)

折叠内容

CustomerNov 122024

评分
Experiment Type: Cell-based assay
Sample: HSPCs
Description: Recombinant TPO (50 ng/mL, Cat. RP00174), IL-3 (15 ng/mL, Cat. RP01362), IL-6 (15 ng/mL, Cat. RP00201) and IL-11 (15 ng/mL, Cat. RP00050) were used to induce HSPCs differentiation into megakaryocytes. After 6 days of induction, the proportion of cells expressed CD41/42+ reached 42.5%.

背景信息

The protein is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells.

基因ID

3589

Swiss Prot

P20809

别名

IL11;AGIF;IL-11

研究领域