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活性验证

Recombinant Human c-Kit ligand/KITLG/SCF Protein (RP00124)

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Immobilized recombinant human SCF at 1 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1-6 ng/mL.

Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 4.47-17.88 ng/mL, corresponding to a specific activity of 5.59×104~2.24×105 units/mg.

Recombinant Human c-Kit ligand/KITLG/SCF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00124
价 格：  620.00
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详细信息

靶点
c-Kit ligand/SCF/KITLG
细胞系
21.89 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
30-40 kDa
产品组成
Glu26-His214
物种
HEK293 cells
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Publishing research using RP00124? Please let us know so that we can cite the reference in this datasheet.

背景信息

The protein is tyrosine-kinase receptor. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.

基因ID
4254
Swiss Prot
P21583
别名
KITLG;DCUA;DFNA69;FPH2;FPHH;KL-1;Kitl;MGF;SCF;SF;SHEP7
研究领域