Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein (RP00630B)

Biotinylated Human IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2 μg/mL (100 μL/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human IL-23R, hFc Tag with the EC₅₀ of 21.0ng/mL determined by ELISA (QC Test).

Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 0.5 μg/mL (100 μL/Well) on streptavidin (5 μg/mL) precoated plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC₅₀ of 2.3ng/mL determined by ELISA.

All(3)|

货号: RP00630B

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

21.7 kDa (IL-23 alpha) , 34.7 kDa (IL-12 beta)

Observed MW

24 kDa (IL-23 alpha) , 40-50 kDa (IL-12 beta)

标签

C-His&Avi

纯度

> 95% by SDS-PAGE

内毒素

< 1 EU/μg of the protein by LAL method

制剂

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

描述

Biotinylated Recombinant Human IL-23/IL-12B&IL-23A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)) of Human IL-23 alpha&IL-12 beta (Accession #Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)) fused with a C-His&Avi tag at the C-terminus.

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！

客户数据及评论 (0)

折叠内容

背景信息

The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.

基因ID

51561&3593

Swiss Prot

Q9NPF7(IL-23 alpha)&P29460(IL-12 beta)

别名

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

研究领域