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Recombinant Human IGF1R/CD221 Protein (RP01143)

Recombinant Human IGF1R/CD221 Protein was determined by SDS-PAGE with Coomassie Blue, showing three bands at approximately 150 kDa, 120 kDa and 48 kDa.

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货号: RP01143
促销价:   ¥2000
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
81 kDa(α chain), 23 kDa(β chain) ,104 kDa(single chain)
Observed MW
120 kDa (α chain), 48 kDa(β chain), 150 kDa(single chain),
标签
C-His
纯度
> 90 % by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution PBS Buffer, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human IGF1R/CD221 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asn932) of human IGF1R (Accession #NP_000866.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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