Recombinant Human IL-23/IL-12B&IL-23A Protein (RP01160)

Recombinant Human IL-23/IL-12B&IL-23A Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 20(IL-23A),45-50(IL-12B) kDa and 65-70 kDa, respectively.

Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/mL.

Recombinant Human IL-23/IL-12B&IL-23A induce IL-17 secretion by mouse splenocytes. The ED₅₀ for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×10⁴~4.71×10⁴ units/mg.

All(3)|

货号: RP01160

详细信息

种属

Human

表达宿主

HEK293 cells

Calculated MW

19.50 kDa(IL-12A), 34.71 kDa(IL-12B)

Observed MW

20 kDa(IL-23A), 45-50 kDa(IL-12B)

标签

No tag(IL12B)&N-his(IL23A)

纯度

> 95% by SDS-PAGE.

内毒素

< 0.1 EU/μg of the protein by LAL method.

制剂

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

描述

Active Recombinant Human IL-12B&IL-23A Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328&Arg20-Pro189) of human IL-23 (Accession #NP_002178.2/NP_057668.1) fused with a 6×His tag at the N-terminus(IL23A).

储存

Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。

生物活性

1.Measured by its binding ability in a functional ELISA.Immobilized Human IL-12B&IL-23A at 5μg/mL (100 μL/well) can bind Human IL23R with a linear range of 4-135 ng/ml.
2.Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED₅₀ for this effect is 21.23-84.94 ng/mL, corresponding to a specific activity of 1.17×10⁴~4.71×10⁴ units/mg.

复溶

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。