产品中心 / 产品类型 / 重组蛋白 / SARS-CoV-2相关蛋白

Biotinylated Recombinant SARS-COV-2 Spike RBD Protein (RP02326)

Biotinylated SARS-COV-2 Spike RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.

Immobilized Human ACE2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated SARS COV-2 Spike RBD, His Tag with the EC50 of 0.097μg/ml determined by ELISA.

All(3)|
货号: RP02326
促销价:   ¥5250
货    期:在线咨询货期
抗体定制服务咨询

文献引用 (1)

折叠内容

Publishing research using RP02326? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
SARS-COV-2
表达宿主
HEK293 cells
描述
Biotinylated Recombinant SARS-COV-2 SARS-COV-2 Spike RBD Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Arg319-Asn532) of SARS-COV-2 SARS-COV-2 Spike RBD fused with His tag and Avi at the C-terminal.
标签
C-His&Avi
纯度
> 95% by Tris-Bis PAGE;> 95% by SEC-HPLC
内毒素
< 1 EU/μg of the protein by LAL method.
生物活性
Immobilized Human ACE2,His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated SARS COV-2 Spike RBD,His Tag with the EC50 of 0.097 μg/mL determined by ELISA.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
=
÷

背景信息

Swiss Prot
别名
S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD
>