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Recombinant Mouse IL-17A/CTLA-8 Protein (RP02520)

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Recombinant Mouse IL-17A/CTLA-8 Protein was determined by SDS-PAGE with Coomassie Blue, showing bands at 17 kDa.

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货号: RP02520
促销价：   ￥1600
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折叠内容

Publishing research using RP02520? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Mouse
表达宿主
E. coli
描述
Recombinant Mouse IL-17A Protein is produced by Escherichia coli expression system. The target protein is expressed with sequence of Mouse IL-17A fused with polyhistidine tag at the C-terminus
标签
C-His
纯度
> 98% by SDS-PAGE.
内毒素
<0.1EU/μg
生物活性
Measured by its ability to inhibit IL-6 secretion in 3T3 cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-17A is > 1 x 106 IU/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.
储存
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).

基因ID
16171
Swiss Prot
Q62386
别名
Il17;Ctla8;IL-17;Ctla-8;IL-17A;IL17A;il17a
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