Western blot analysis of lysates from wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells using PD-L1/CD274 Rabbit mAb (A19135) at 1:1000 dilution.wild type (WT) and PD-L1/CD274 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37℃ for 48 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using PD-L1/CD274 Rabbit mAb (A19135) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit mAb (A19135) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using PD-L1/CD274 Rabbit mAb (A19135) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABflo® 488 Rabbit anti-Human CD86 mAb
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Flow cytometry:1X10^6 K-562 cells (negative control,left) and Raji cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD86 mAb(A21940,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
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Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,2 μg/mL,left) or ABflo® 488 Rabbit anti-Human CD86 mAb (A21940,2 μg/mL,right). Cells in the monocyte gate were used for analysis.
ABflo® 488 Rabbit anti-Human PD-L1/CD274 mAb
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Flow cytometry: 1X10^6 K-562 cells (negative control,left) and MDA-MB-231 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human PD-L1/CD274 mAb (A22304,2 μg/mL,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,2 μg/mL,blue line). Non-fluorescently stained cells was used as blank control (red line).
ABflo® 488 Rabbit anti-Human CD27 mAb
Flow cytometry:1X10^6 HEL cells (negative control,left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit anti-Human CD27 mAb(A22063,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).
Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063,5 μl/Test,right).
Flow cytometry:1X10^6 Human PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063,5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009，5 μl/Test).
Flow cytometry:1X10^6 Monkey PBMC cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test) or ABflo® 488 Rabbit anti-Human CD27 mAb(A22063,5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009，5 μl/Test).
ABflo® 488 Rabbit anti-Human CD28 mAb
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Flow cytometry:1X10^6 K-562 cells(negative control,left) and U266 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD28 mAb(A23405,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line).Non-fluorescently stained cells was used as blank control (red line).
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Flow cytometry:1X10^6 U266 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD28 mAb(A23405,5 μl/Test,right).
PE Rabbit anti-Human CD152/CTLA-4 mAb
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Flow cytometry: 1X10^6 293F cells(negative control,left) and 293F(Transfection,right) cells were surface-stained with PE Rabbit anti-Human CD152/CTLA-4 mAb(A23713,5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line).Non-fluorescently stained cells was used as blank control (red line).
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Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human CD152/CTLA-4 mAb(A23713,5 μl/Test,right).
ABflo® 594 Rabbit anti-Human CD47 mAb
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Flow cytometry:1X10^6 HepG2 cells (Low Expression, Left) and MOLT-4 cells(Right) were surface-stained with ABflo® 594 Rabbit anti-Human CD47 mAb(A24302,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
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Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Human CD47 mAb(A24302,5 μl/Test,right).
ABflo® 594 Rabbit anti-Mouse CD47 mAb
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Flow cytometry: 1X10^6 C57 BL/6 mouse Splenocytes were surface-stained with ABflo® 594 Rabbit anti-Mouse CD47 mAb (A24379,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained C57 BL/6 mouse Splenocytes were used as blank control (red line).
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Flow cytometry: 1X10^6 Hep G2 cells (negative control) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD47 mAb (A24379,5 μl/Test,orange line) or ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,blue line). Non-fluorescently stained Hep G2 cells were used as blank control (red line).
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Flow cytometry: 1X10^6 C57 BL/6 mouse Splenocytes were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821,5 μl/Test,left) or ABflo® 594 Rabbit anti-Mouse CD47 mAb (A24379,5 μl/Test,right).
