Western blot analysis of various lysates, using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.
Immunohistochemistry analysis of EIF4A1/EIF4A2/EIF4A3 in paraffin-embedded human kidney using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of EIF4A1/EIF4A2/EIF4A3 in paraffin-embedded mouse kidney using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of EIF4A1/EIF4A2/EIF4A3 in paraffin-embedded rat lung using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF7 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
