Recombinant Human R-spondin-1/RSPO1 Protein (RP00071)

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Recombinant Human R-spondin-1/RSPO1 Protein (RP00071)

Human gastric cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), FGF10(Cat. RP01140), RSPO1(Cat. RP00071), Wnt-3a Surrogate(Cat. RP01618SLQ).

The purity of Recombinant Human R-spondin-1/RSPO1 Protein is greater than 90% as determined by SEC-HPLC.

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Human stomach organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Recombinant Human R-spondin-1/RSPO1 Protein induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 6.61~26.44 ng/mL in the presence of 5 ng/mL Wnt Surrogate Protein, corresponding to a specific activity of 3.78×10⁴~1.51×10⁵ units/mg.

Recombinant Human R-spondin-1/RSPO1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse small intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071).

Human colorectal cancer organoid were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237)， FGF10(Cat. RP01140), RSPO1(Cat. RP00071), HGF(Cat. RP01602).

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

Mouse lung organoids were cultured in Matrigel (Cat. RPM0003) with medium supplemented with EGF (Cat. RP03287), Noggin (Cat. RP01237), RSPO1 (Cat. RP00071), Wnt-3a (Cat. RP01618SLQ), and FGF-10 (Cat. RP01140).

All(11)|

货号: RP00071

详细信息

靶点

R-spondin-1

细胞系

26.44 kDa

突变描述

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.

敲除验证

39 kDa

产品组成

Arg31-Ala263

物种

HEK293 cells

保存条件

Store at -20℃

运输条件

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

客户数据及评论 (8)

折叠内容

中国科学院动物研究所Nov 282025

评分
Experiment Type: Cell-based assay
Sample: hPSC
Description: The differentiation medium containing RSPO1 (500 ng/mL), Noggin (100 ng/mL), and EGF (100 ng/mL) was used to induce the differentiation of mid-hindgut cells into small intestinal organoids. After 7 days of induction, the expression of markers CDX2, MUC2, CgA, and E-Cad was assessed by immunofluorescence.
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厦门客户Sep 042025

评分
Experiment Type: Cell-based assay
Sample: mouse small intestinal organoids, mouse bile duct organoids, mouse colonic organoids, and human colorectal cancer organoids
Description: RSPO1 demonstrates comparable efficacy to controls in the cultivation of mouse small intestinal organoids, mouse biliary duct organoids, mouse colonic organoids, and human colorectal cancer organoids, with equivalent organoid growth outcomes and phenotypic status.
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厦门百脑慧医学研究有限公司Jul 282025

评分
Experiment Type: Cell-based Assay
Sample: prostate cancer cells
Description: The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).
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南京农业大学Jun 112025

评分
Experiment Type: Cell-based assay
Sample: tissue
Description: Animal tissues-derived intestinal stem cells were cultured in organoid culture medium containing factor combinations (WNT-3A, Cat. RP01618+EGF, Cat. RP01030+R-spondin-1, Cat. RP01237+Noggin, Cat. RP00071+ matrix gel, Cat. RPM0003), and then small intestinal organoids were formed.
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厦门大学Nov 122024

评分
Experiment Type: Cell-based assay
Sample: Intestinal crypts
Description: Mouse intestinal crypts were cultured with organoid culture medium supplemented with recombinant human NOG (100 ng/mL, Cat. RP01237) and R-spondin-1 (500 ng/mL, Cat. RP00071) for 132 h and 144 h. And mouse intestinal organoid were obtained.
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厦门大学Nov 122024

评分
Experiment Type: Cell-based assay
Sample: 293T
Description: Different concentration (100 ng/mL, 300 ng/mL, 500 ng/mL) of R-spondin-1 (Cat. RP00071) were used to treat 293T cells for 6 h. The expression of related gene was detected by luciferase assay to reflect the potency of R-spondin-1 enhancing Wnt pathway signal. At the concentration of 300 ng/mL, the potency of R-spondin-1 reached the highest, and the wnt pathway signal was enhanced by 3.1 times compared with the wnt3a stimulation alone.
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复旦大学Nov 122024

评分
Experiment Type: Cell-based assay
Sample: Cell
Description: The picture shows the culture results of mESo (mouse esophageal organoids). R-Spondin1 (Cat. RP00071), Noggin (Cat. RP01237) and EGF (Cat. RP01502) were used during the organoids culture and the final concentration were 200，100，50 ng/mL respectively.
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中国中医科学院医学实验中心Oct 232024

评分
Experiment Type: Cell-based assay
Sample: tumor tissue
Description: Patient tissue-derived kidney tumor cells were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spondin-1, Cat. RP00071 + 20 ng/mL FGF-10, Cat. RP01140 + 50 ng/mL EGF, Cat. RP01030), and then kidney tumor organoids were formed.
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背景信息

This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

基因ID

284654

Swiss Prot

Q2MKA7

别名

RSPO1;CRISTIN3;RSPO