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活性验证

Recombinant SARS-CoV-2 Nucleocapsid Protein (RP01264LQ)

Recombinant SARS-CoV-2 Nucleocapsid Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55 kDa.

The purity of SARS-COV-2 Nucleocapsid Protein with His tag (Cat.RP01264) was greater than 95% as determined by SEC-HPLC.

Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL.

Immobilized Recombinant SARS-CoV-2 envelope Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.

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货号: RP01264LQ
促销价:   ¥2400
货    期:现货产品
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详细信息

种属
SARS-CoV-2
表达宿主
E. coli
Calculated MW
46.48 kDa
Observed MW
55 kDa
标签
N-His
纯度
> 95% by SDS-PAGE;> 95% by HPLC.
内毒素
< 1.0 EU/μg of the protein by LAL method.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.Contact us for customized product form or formulation.
描述
Recombinant SARS-CoV-2(2019-nCoV) Nucleocapsid Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala419) of SARS-COV-2(2019-nCoV) Nucleocapsid (Accession #QHD43423.2) fused with an 6×His tag at the N-terminus.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.

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Publishing research using RP01264LQ? Please let us know so that we can cite the reference in this datasheet.

    背景信息

    基因ID
    别名
    Nucleoprotein
    >