纯度
≥ 95 % as determined by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant SARS-CoV-2 S1+S2 ECD(S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of SARS-COV-2 S1+S2 ECD(S-ECD) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.It is the wildtype sequence in pre-fusion state without any mutation.
储存
Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 S1+S2 ECD(S-ECD) at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.72 ng/mL.
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!