首页 / 产品和服务 / 专题推荐 / 外泌体研究专题 / 外泌体表征鉴定相关通用标志物
细胞外囊泡(Extracellular Vesicle, EV)，包括外泌体，微囊泡、微粒、凋亡小体和其他EV亚群，目前在技术上获得一个不含非囊泡成分的纯EV组分用于功能性研究是具有挑战性的。《MISEV 2018》更新了囊泡研究所需最小的生化、生物物理和功能标准文件[1]，用于对细胞外囊泡及其内含物的鉴定和功能分析进行规范。关于外泌体表征鉴定，需包含如下方面：
1）至少包含3个EVs阳性蛋白标志物鉴定
—跨膜或脂质结合蛋白（如CD9,CD63,CD81等）
—胞质蛋白（TSG101,Alix等）
2）至少1个EVs阴性蛋白标志物鉴定（根据样本类型选择）
1）纳米颗粒跟踪分析（NTA）或纳米流式
| 候选靶点[2-3] | 产品货号 | 产品名称 | 产品应用 | 反应种属 |
|---|---|---|---|---|
| HSPA8/Hsc70 | A0415 | Hsc70/HSPA8 Rabbit mAb | WB | Human, Mouse, Rat |
| A14001 | Hsc70/HSPA8 Rabbit pAb | WB, IF | Human, Mouse, Rat | |
| A2487 | Hsc70/HSPA8 Rabbit pAb | WB, IHC,IF | Human, Mouse, Rat, Monkey | |
| HSP90AA1/Hsp90α | A5006 | Hsp90α Rabbit mAb | WB, IP | Human, Mouse, Rat |
| A0365 | Hsp90α Rabbit pAb | WB, IF | Human, Mouse, Rat, Monkey | |
| HSP90AB1/Hsp90β | A19574 | Hsp90β Rabbit mAb | WB, IF | Human, Mouse, Rat |
| A18015 | [KO Validated] Hsp90β Rabbit pAb | WB, IHC, IF | Human, Mouse, Rat, Monkey | |
| CD9 | A19027 | CD9 Rabbit mAb | WB, IHC, IP | Human, Mouse |
| A1703 | CD9 Rabbit pAb | WB, IHC | Human, Mouse, Rat | |
| TSG101 | A5789 | TSG101/VPS23 Rabbit mAb | WB | Human, Rat |
| ALIX | A2215 | ALIX / PDCD6IP Rabbit pAb | WB, IF | Human, Mouse, Rat |
| CD63 | A19023 | CD63 Rabbit mAb | WB | Human |
| A21920 | [KO Validated] CD63 Rabbit mAb | WB | Human | |
| CD81 | A4863 | CD81 Rabbit mAb | WB | Mouse, Rat |
| A5270 | CD81 Rabbit pAb | WB, IHC,IF | Human, Mouse, Rat | |
| FLOT2 | A6590 | FLOT2 Rabbit pAb | WB | Human, Mouse, Rat |
| FLOT1 | A6220 | [KO Validated] Flotillin 1 Rabbit pAb | WB, IHC, IF, IP | Human, Mouse, Rat |
| A3023 | Flotillin 1 Rabbit mAb | WB, IHC | Human, Mouse, Rat | |
| HSPA4/Hsp70 | A19705 | HSPA4 Rabbit mAb | WB,IHC | Human,Mouse,Rat |
| ACTB | AC026 | β-Actin Rabbit mAb (High Dilution) | WB | Human, Mouse, Rat, Chicken, Zebrafish, Pig |
| AC038 | ACTB Rabbit mAb | WB | Human, Mouse, Rat, Chicken, Zebrafish, Pig | |
| RAP1B | A12925 | RAP1B Rabbit pAb | WB, IF | Human, Mouse |
| Moesin | A4915 | Moesin Rabbit mAb | WB, IHC | Human, Mouse, Rat |
| A2M | A9752 | Alpha-2-Macroglobulin (A2M) Rabbit mAb | WB,IHC,IF | Human,Mouse,Rat |
| A1573 | Alpha-2-Macroglobulin (A2M) Rabbit pAb | WB | Human,Mouse,Rat | |
| Gelsolin | A2612 | Gelsolin Rabbit mAb | WB,IHC | Human,Mouse |
| A22184 | [KD Validated] Gelsolin Rabbit mAb | WB,IHC | Human | |
| A16869 | Gelsolin Rabbit pAb | WB,IHC,IF | Human,Mouse,Rat | |
| B2M | A11642 | beta 2 Microglobulin Rabbit mAb | WB,IHC | Human,Mouse,Rat |
| A1562 | beta 2 Microglobulin Rabbit pAb | WB,IHC | Human,Mouse,Rat | |
| PRDX2 | A4308 | Peroxiredoxin 2 (PRDX2) Rabbit mAb | WB | Human,Mouse,Rat |
| A1919 | [KO Validated] Peroxiredoxin 2 (PRDX2) Rabbit pAb | WB,IF | Human,Mouse,Rat | |
| Filamin A | A0927 | Filamin A Rabbit pAb | WB,IP | Human,Mouse |
| A16376 | Filamin A Rabbit pAb | WB,IHC,IF | Human,Mouse,Rat | |
| A3738 | Filamin A Rabbit mAb | WB,IHC | Human,Mouse,Rat | |
| A7896 | Filamin A Rabbit pAb | WB,IHC | Human,Mouse,Rat | |
| Syntenin | A5497 | Syntenin Rabbit mAb | WB, IHC, IF | Human, Mouse, Rat |
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