单细胞及微量珍稀样本的甲基化研究主要应用于疾病发生机制，胚胎植入前诊断，胚胎早期发育，生殖细胞重组，干细胞及细胞异质性等研究领域；在肿瘤早筛检测中，ctDNA的甲基化越来越受到国内外多家癌症早筛公司的青睐。然而微量样本的DNA甲基化组学研究很大程度上受制于建库测序技术。
ABclonal 微量样本DNA甲基化测序解决方案
ABclonal拥有完整、丰富的产品线，可提供酶法转化与建库的关键原料与试剂，还可以提供常用的NGS DNA聚合酶和纯化磁珠。
微量样本DNA甲基化测序研究工具
| 应用 | 产品名称 | 简介 | 货号 |
|---|---|---|---|
| 酶原料 | TET2 | 增强型双加氧酶，甲基化研究的工具酶 | RK20557 |
| T4-BGT | 糖基转移酶，甲基化研究的工具酶 | RK20558 | |
| APOBEC | 胞嘧啶脱氨酶，甲基化研究的工具酶 | RK20559 | |
| 盐法转化 | DNA Methylation Bisulfite Kit | DNA甲基化BS转化试剂盒（柱法） | RK30203 |
| 甲基化qPCR | MethyLight 2X qPCR Probe Master Mix | DNA甲基化qPCR反应的专用试剂，MSP分析 | RK21240 |
| 甲基化DNA建库 | Scale Methyl-DNA Lib Prep Kit for Illumina | 通用型、快速、高效，适用于Illumina 平台 | RK20220 |
| DNA甲基化酶Mix / Kit | Gloria U 2X HS Master Mix | 可有效扩增含尿嘧啶的模板 | RK20722 |
| Gloria U 2X Mix for NGS | 可用于含有尿嘧啶的模板扩增或者含有尿嘧啶的文库扩增 | RK20723 | |
| 磁珠 | AFTMag NGS DNA Clean Beads | 核酸纯化，片段筛选 | RK20257 |
组蛋白经过不同形式的翻译后修饰（PTM），致使其与 DNA 的相互作用受到影响。一些修饰破坏了组蛋白-DNA相互作用，导致核小体解旋，修饰异常直接导致染色质结构松散、基因表达失调并引发各类疾病， 例如神经性疾病、心血管疾病、慢性肾脏疾病以及免疫性疾病等。目前已知类型的组蛋白修饰至少有 9 种。乙酰化、甲基化、磷酸化和泛素化是大家最了解的，而N-乙酰葡萄糖胺糖基化、瓜氨酸化、巴豆酰化和异构化是后期才发现的修饰。每种修饰都是通过一组特定的酶将修饰基团添加到组蛋白氨基酸残基上或从组蛋白氨基酸残基上去除的。
组蛋白修饰抗体产品列表
| 靶点 | 产品名称 | 应用 | 货号 |
|---|---|---|---|
| Histone | Histone H1.0 Rabbit mAb | ELISA, WB, IHC-P | A4342 |
| Histone | Histone H2A Rabbit mAb | ELISA, WB, IHC-P, IP | A3692 |
| Histone | Histone H2AX Rabbit mAb | ELISA, WB, IHC-P, ChIP | A11412 |
| Histone | Histone H3 Rabbit mAb | ELISA, WB, IHC-P, IF/ICC, CUT&Tag | A22348 |
| Histone | Histone H3.3 Rabbit mAb | ELISA, WB, IHC-P, ChIP, ChIP-seq | A4835 |
| Histone | Histone H4 Rabbit mAb | ELISA, WB, IHC-P, IF/ICC, ChIP, ChIP-seq | A23000 |
| Histone | Histone H4 Rabbit mAb | ELISA, WB, IHC-P | A19815 |
| Acetyl-Histone | Acetyl-Histone H3-K9 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, IP, ChIP, CUT&Tag, ChIP-seq | A21107 |
| Acetyl-Histone | Acetyl-Histone H3-K9 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC,ChIP | A22567 |
| Acetyl-Histone | Acetyl-Histone H3-K18 Rabbit mAb | ELISA, DB, WB, IHC-P, ChIP, CUT&Tag, ChIP-seq | A20735 |
| Acetyl-Histone | Acetyl-Histone H3-K18 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC,ChIP | A22566 |
| Acetyl-Histone | Acetyl-Histone H3-K23 Rabbit mAb | ELISA, WB, IF/ICC | A2770 |
| Acetyl-Histone | Acetyl-Histone H3-K27 Rabbit mAb | ELISA, DB, WB, IHC-P, IP, IF/ICC, ChIP | A2771 |
| Acetyl-Histone | Acetyl-Histone H3-K56 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, ChIP, ChIP-seq, CUT&Tag | A22565 |
| Acetyl-Histone | Acetyl-Histone H4-K5 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC | A19525 |
| Acetyl-Histone | Acetyl-Histone H4-K5 Rabbit mAb | ELISA, WB, IHC-P, ChIP, ChIP-seq, CUT&Tag | A23080 |
| Acetyl-Histone | Acetyl-Histone H4-K12 Rabbit mAb | ELISA, DB, WB, IHC-P, ChIP, ChIP-seq | A22754 |
| Acetyl-Histone | Acetyl-Histone H4-K16 Rabbit mAb | ELISA, DB, WB, IHC-P, ChIP | A23091 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K4 Rabbit mAb | ELISA, DB, WB, IF/ICC, CUT&Tag | A22078 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K9 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, ChIP, CUT&Tag | A20734 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K18 Rabbit mAb | ELISA, DB, WB, IHC-P, IP, IF/ICC, ChIP, CUT&Tag | A20680 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K27 Rabbit mAb | ELISA, DB, WB, IF/ICC | A22170 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K36 Rabbit mAb | ELISA, DB, WB, IHC-P | A22863 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K79 Rabbit mAb | ELISA, DB, WB, IHC-P, ChIP, ChIP-seq | A21223 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-R2 Rabbit mAb | ELISA, DB, WB, CUT&Tag | A19645 |
| MonoMethyl-Histone | MonoMethyl-Histone H4-K20 Rabbit mAb | ELISA, DB, WB, IHC-P | A22572 |
| DiMethyl-Histone | DiMethyl-Histone H3-K4 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, IP, ChIP, ChIP-seq, CUT&Tag | A22143 |
| DiMethyl-Histone | DiMethyl-Histone H3-K36 Rabbit mAb | ELISA, DB, WB, IHC-P | A22221 |
| DiMethyl-Histone | DiMethyl-Histone H3-K36 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, IP | A22087 |
| DiMethyl-Histone | DiMethyl-Histone H3-K79 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, ChIP, IP, ChIP-seq | A22086 |
| DiMethyl-Histone | DiMethyl-Histone H3-K79 Rabbit mAb | ELISA, DB, WB, ChIP, ChIP-seq | A22142 |
| DiMethyl-Histone | DiMethyl-Histone H4-K20 Rabbit mAb | ELISA, DB, WB, IHC-P | A22269 |
| DiMethyl-Histone | DiMethyl-Histone H4-K20 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC | A22268 |
| Symmetric DiMethyl-Histone | Symmetric DiMethyl-Histone H3-R8 Rabbit mAb | ELISA, WB, DB, IHC-P, IF/ICC | A21207 |
| Symmetric DiMethyl-Histone | Symmetric DiMethyl-Histone H3-R17 Rabbit mAb | ELISA, DB, WB, IHC-P | A22291 |
| TriMethyl-Histone | TriMethyl-Histone H3-K4 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, ChIP | A22225 |
| TriMethyl-Histone | TriMethyl-Histone H3-K4 Rabbit mAb | ELISA, DB, WB, IHC-P, IF/ICC, ChIP, CUT&Tag, ChIP-seq | A22146 |
| TriMethyl-Histone | TriMethyl-Histone H3-K9 Rabbit mAb | ELISA, WB, IF/ICC, ChIP, ChIP-seq, DB, CUT&Tag | A22295 |
| TriMethyl-Histone | TriMethyl-Histone H3-K27 Rabbit mAb | ELISA, WB, IHC-P, IF/ICC, ChIP，CUT&Tag | A22396 |
| TriMethyl-Histone | TriMethyl-Histone H3-K27 Rabbit mAb | ELISA, DB, WB, IF/ICC, IP, ChIP, CUT&Tag | A22006 |
| TriMethyl-Histone | TriMethyl-Histone H3-K36 Rabbit mAb | ELISA, DB, WB, IP, IHC-P, ChIP, ChIP-seq | A20379 |
| Formyl-Histone | Formyl-Histone H2B-K120 Rabbit mAb | ELISA, WB, IHC-P | A0065 |
| Phospho-Histone | Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb | ELISA, WB | AP1132 |
| Phospho-Histone | Phospho-Histone H2AX-S139 Rabbit mAb | ELISA, WB, IHC-P, IF/ICC | AP0687 |
| Phospho-Histone | Phospho-Histone H3.3-T3 Rabbit mAb | ELISA, WB | AP1152 |
| Phospho-Histone | Phospho-Histone H3-S10 Rabbit mAb | ELISA, WB, IHC-P, IF/ICC | AP0002 |
