RNA表达鉴定分析

转录组是鉴定表达分析特定功能RNA分子的重要手段,从基本的RNA表达分析,到转录组测序需要采用不同的建库方式。

产品列表

产品名称 简介 货号
Poly(A) mRNA Capture Module 适合于从纯化的 total RNA 中分离获取 poly(A) RNA RK20340
rRNA Depletion module (H / M / R) 适用于人 / 大、小鼠total RNA及发生降解的RNA样本 RK20348
Fast RNA-seq Lib Prep Kit V2 缩短建库时间至3 hr, 兼容 MGI 、Illumina 平台 RK20306
RNA-seq Lib Prep Kit(with FS) 片段化酶切打断RNA建库 RK20308
Stranded mRNA-seq Lib Prep Module for Illumina 适合于 Illumina 平台 RK20349
mRNA-seq Lib Prep Module for Illumina 适合于 Illumina 平台 RK20350
Small RNA Lib Prep Kit for Illumina V3 适合于 Illumina 平台 RK20312
Single Cell / Low Input cDNA Synthesis & Amplification Module 适用于单细胞全长cDNA合成 RK20310
First Strand Synthesis Module 非链特异性cDNA一链合成试剂盒 RK20353
First Strand Synthesis Module (Stranded) 链特异性cDNA一链合成试剂盒 RK20342
Second Strand Synthesis Module 非链特异性cDNA二链合成试剂盒 RK20346
Second Strand Synthesis Module (Stranded) 链特异性cDNA一链合成试剂盒 RK20343

RNA-蛋白复合体研究

对RNA-蛋白复合体进行研究,有两种常见的研究方法:
用特定RNA结合蛋白(RBP)的抗体进行RNA pull down捕获并富集其结合的RNA,利用测序或RT-qPCR等方法对捕获的RNA进行鉴定和分析;使用体外转录法获得生物素标记的RNA,与蛋白提取物共孵育,在体外形成RNA-蛋白质复合物,然后用链霉亲和素标记的磁珠捕获并富集其结合的RNA-蛋白复合物。洗脱后,利用质谱或western blot鉴定或分析相关RNA结合蛋白(RBP)。

RBP抗体产品列表

产品名称 应用 物种 货号
[KD Validated] Calreticulin Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A20986
[KD Validated] Calreticulin Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A20986
[KD Validated] NQO1 Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A19586
[KO Validated] Fatty Acid Synthase (FASN) Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A21182
[KO Validated] HMGB1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A19529
[KO Validated] HuR/ELAVL1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP Human, Mouse, Rat A19622
[KO Validated] SUMO1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A19121
ADAR1 Rabbit mAb ELISA, WB Human A11466
Annexin A2 Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A11235
Argonaute-2 Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A19709
ATP5A1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP Human, Mouse, Rat A11217
Cytokeratin 18 (KRT18) Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A19778
DDX5 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP Human, Mouse, Rat A11339
DDX6 Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A9634
DHX9/RNA Helicase A Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP Human, Mouse, Rat A4563
eIF2α Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A21221
eIF4A3 Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A8985
EWSR1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC,IP Human, Mouse, Rat A9640
Fibrillarin/U3 RNP Rabbit mAb ELISA, WB, IF/ICC, IP Human, Mouse, Rat A0850
FMRP Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A4539
FSP1/S100A4 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A19109
G3BP1 Rabbit mAb ELISA, WB, IF/ICC, IP Human, Mouse, Rat A3968
Galectin 3/LGALS3 Rabbit mAb ELISA, WB, IHC-P, IP Human, Mouse, Rat A11198
HMGB2 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP, ChIP, CUT&Tag Human, Mouse, Rat A9168
hnRNP A1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP Human, Mouse, Rat A11564
HSP27/HSPB1 Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A11156
HSP60/HSPD1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A0564
Hsp90β Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A23489
ILF3 Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A8186
Ku70 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse A11223
NAT10 Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A19286
NONO/p54nrb Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A3800
P4HB Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A19239
PARP1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A19596
PTBP1 Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A3487
S6 Ribosomal Protein (RPS6) Rabbit mAb ELISA, WB, IF/ICC Human, Mouse, Rat A11874
SRSF1/SF2/ASF Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A4091
STIP1 Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A0036
TDP-43/TARDBP Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP, ChIP Human, Mouse, Rat A19123
TFAM Rabbit mAb ELISA, WB, IHC-P, IF/ICC Human, Mouse, Rat A3173
UBE2I Rabbit mAb ELISA, WB, IHC-P Human, Mouse, Rat A4396
UPF1/RENT1 Rabbit mAb ELISA, WB,IF/ICC, IP Human, Mouse, Rat A5071

热门抗体

RNA-蛋白复合体研究产品列表

RNA修饰

RNA的修饰对RNA的定位、活性、功能及稳定性等方面均有不同影响。研究RNA在不同生理及病理条件下修饰的动态变化,有助于我们更深刻的认识特定RNA的作用和调节机理。目前,RNA甲基化是研究热点,RNA最常见的内部修饰包括了N6-腺苷酸甲基化(m6A)、N1-腺苷酸甲基化(m1A)、胞嘧啶羟基化(m5C)等。其中,N6-甲基腺嘌呤(m6A)研究最为深入和广泛,多种类型RNA的m6A甲基化修饰在基因调控、干细胞分化、癌细胞增殖、组织生长发育等过程中起关键作用。

RNA修饰抗体产品列表

分类 产品名称 应用 货号
RNA修饰 N6-methyladenosine / m6A Rabbit mAb ELISA, Nucleotide Array, DB, meRIP, IF/ICC A19841
RNA修饰 N6-methyladenosine / m6A Rabbit mAb ELISA, Nucleotide Array, DB, IF/ICC, meRIP A22411
书写器 METTL3 Rabbit mAb ELISA, WB, IF/ICC, IP A19079
书写器 METTL4 Rabbit mAb ELISA, IHC-P A23539
书写器 RBM3 Rabbit mAb ELISA, WB, IHC-P, IF/ICC A20911
书写器 RBMXL2 Rabbit mAb ELISA, IF/ICC A21075
擦除器 ALKBH5 Rabbit mAb ELISA,WB,IHC-P,IP A22137
擦除器 [KO Validated] FTO Rabbit mAb ELISA, WB, IHC-P A20992
擦除器 FTO Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP A3861
阅读器 eIF3e Rabbit mAb ELISA, WB, IHC-P A3431
阅读器 eIF3B Rabbit mAb ELISA, WB, IF/ICC A9143
阅读器 FMRP Rabbit mAb ELISA, WB, IHC-P A4539
阅读器 hnRNP C Rabbit mAb ELISA, WB, IF/ICC A8958
阅读器 hnRNP E1/PCBP1 Rabbit mAb ELISA, WB A19276
阅读器 hnRNP E1/PCBP1 Rabbit mAb ELISA, WB A22141
阅读器 hnRNP E2/PCBP2 Rabbit mAb ELISA, WB, IP A21119
阅读器 IGF2BP1/IMP1 Rabbit mAb ELISA, WB, IF/ICC A22612
阅读器 [KO Validated] IGF2BP1/IMP1 Rabbit pAb ELISA, WB A22246
阅读器 IGF2BP2/IMP2 Rabbit mAb ELISA, WB, IHC-P, IP A5189
阅读器 IGF2BP3/IMP3 Rabbit mAb ELISA, WB, IHC-P, IF/ICC, IP A23295
阅读器 YTHDF1 Rabbit mAb ELISA, WB A23773

热门抗体