Immunohistochemistry analysis of ATG3 in paraffin-embedded human kidney tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded human liver tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded human placenta tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded mouse kidney tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded human tonsil tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded rat liver tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of ATG3 in paraffin-embedded mouse kidney tissue using ATG3 Rabbit mAb (A19594) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Western blot analysis of lysates from wild type(WT) and ATG7 knockdown (KD) HeLa cells, using [KD Validated] ATG7 Rabbit mAb (A19604) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: ARC0083.

Immunohistochemistry analysis of paraffin-embedded rat spleen using [KD Validated] ATG7 Rabbit mAb (A19604) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using [KD Validated] ATG7 Rabbit mAb (A19604) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of lysates from wild type(WT) and LC3B knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution.wild type(WT) and LC3B knockdown (KD) 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded human brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Confocal imaging of HeLa cells (treated with chloroquine) and HeLa cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665,dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KD Validated] LC3B Rabbit mAb (A19665). Western blot was performed from the immunoprecipitate using LC3B antibody (A19665) at a dilution of 1:1000.

Western blot analysis of various lysates using [KO Validated] ATG5 Rabbit mAb (A19677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human placenta using [KO Validated] ATG5 Rabbit mAb (A19677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KO Validated] ATG5 Rabbit mAb (A19677). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit mAb (A19677) at a dilution of 1:1000.

Western blot analysis of lysates from PC-12 cells, using SQSTM1/p62 Rabbit mAb (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from HeLa cells, using SQSTM1/p62 Rabbit mAb (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human colon carcinoma tissue using SQSTM1/p62 Rabbit mAb (A19700) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded Human lung adenocarcinoma tissue using SQSTM1/p62 Rabbit mAb (A19700) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human liver cancer tissue using SQSTM1/p62 Rabbit mAb (A19700) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human cervix cancer tissue using SQSTM1/p62 Rabbit mAb (A19700) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human small intestine tissue using SQSTM1/p62 Rabbit mAb (A19700) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of HeLa cells using SQSTM1/p62 Rabbit mAb (A19700, dilution 1:200) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg SQSTM1/p62 Rabbit mAb (A19700). Western blot was performed from the immunoprecipitate using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at a dilition of 1:1000.

Immunohistochemistry analysis of paraffin-embedded human tonsil using Bcl-2 Rabbit mAb (A19693) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human follicular lymphoma using Bcl-2 Rabbit mAb (A19693) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human appendix using Bcl-2 Rabbit mAb (A19693) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200 μg extracts of Mouse lung using 3 μg Bcl-2 antibody (A19693). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A19693) at a dilution of 1:1000.

Western blot analysis of extracts of Rat lung, using Beclin 1 antibody (A21191) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Western blot analysis of extracts from wild type (WT) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (A21191) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Confocal imaging of HeLa cells using [KO Validated] Beclin 1 Rabbit mAb (A21191, dilution 1:100) (Red). The cells were counterstained with [KO Validated] Vimentin Rabbit mAb (A19607, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Western blot analysis of various lysates, using ULK1 Rabbit pAb (A8529) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Immunofluorescence analysis of HeLa cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.