染色质免疫共沉淀技术（Chromatin Immunoprecipitation，ChIP）也称结合位点分析法，是研究体内蛋白质与DNA相互作用的有力工具，通常用于转录因子结合位点或组蛋白特异性修饰位点的研究。将ChIP与第二代测序技术相结合的ChIP-Seq技术，能够高效地在全基因组范围内检测与组蛋白、转录因子等互作的DNA区段，是表观遗传学研究常用技术。
ChIP-Seq全流程解决方案
ChIP-Seq产品列表
ChIP-Seq验证抗体产品列表
| 靶点 | 产品名称 | 物种 | 货号 |
|---|---|---|---|
| Histone | Histone H3.3 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A4835 |
| Histone | Histone H4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A23000 |
| Acetyl-Histone | Acetyl-Histone H4-K5 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A23080 |
| Acetyl-Histone | Acetyl-Histone H3-K9 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A21107 |
| Acetyl-Histone | Acetyl-Histone H4-K12 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22754 |
| Acetyl-Histone | Acetyl-Histone H3-K18 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A20735 |
| Acetyl-Histone | Acetyl-Histone H3-K27 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22077 |
| Acetyl-Histone | Acetyl-Histone H3-K56 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22565 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K79 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A21223 |
| DiMethyl-Histone | DiMethyl-Histone H3-K4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22143 |
| DiMethyl-Histone | DiMethyl-Histone H3-K79 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22086 |
| DiMethyl-Histone | DiMethyl-Histone H3-K79 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22142 |
| TriMethyl-Histone | TriMethyl-Histone H3-K4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22146 |
| TriMethyl-Histone | TriMethyl-Histone H3-K9 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22295 |
| TriMethyl-Histone | TriMethyl-Histone H3-K36 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A20379 |
| CTCF | CTCF Rabbit mAb | Human, Mouse, Rat | A19588 |
| DNMT3B | DNMT3B Rabbit mAb | Human, Mouse | A22658 |
| GATA3 | GATA3 Rabbit mAb | Human, Mouse | A19636 |
| HMGA1 | HMGA1 Rabbit mAb | Human, Mouse, Rat | A4343 |
| POLR2A | Phospho-POLR2A CTD-S2 Rabbit mAb | Human, Mouse, Rat | AP0996 |
| Rad21 | Rad21 Rabbit mAb | Human, Mouse, Rat | A19749 |
| RXRα | RXRα Rabbit mAb | Human, Mouse, Rat | A19105 |
| SP1 | SP1 Rabbit mAb | Human, Mouse, Rat | A19649 |
| STAT1 | STAT1 Rabbit mAb | Human, Mouse, Rat | A19563 |
CUT&Tag (Cleavage UnderTargets and Tagmentation)的实验原理：通过刀豆蛋白A包被的磁珠(ConA beads)吸附细胞(与细胞膜上的糖蛋白结合)，使用洋地黄皂苷在细胞膜上”打孔”，pA-Tn5识别抗体引且精准靶向目的蛋白。转座酶对目的蛋白附近的DNA进行片段化的同时会加上二代测序所需的部分接头序列，经过PCR扩增即可获得直接用于测序的文库。相较于ChIP-seq技术，CUT&Tag所需的细胞起始量低(10万以下，甚至单细胞)、操作简单(不需要交联、超声片段化及免疫沉淀)、实验周期短、数据信噪比高、重复性好等优势，成为众多表观遗传学研究者的新选择。
CUT&Tag全流程解决方案
| 表观遗传学研究技术 | 解决方案 | 产品名称 |
|---|---|---|
| CUT&Tag | CUT&Tag试剂盒 | CUT&Tag Assay Kit (pAG-Tn5) for illumina |
| CUT&Tag验证抗体 | ||
| 配套产品 | pAG-Tn5 Transposome | |
| pAG-Tn5 Transposase for CUT&Tag | ||
| AFTMag NGS DNA Clean Beads | ||
| 0.2 mL (24-well) Magnetic Separation Device Plus | ||
CUT&Tag相关产品列表
| 应用 | 货号 | 产品名称 | 简介 |
|---|---|---|---|
| CUT&Tag 试剂盒 | RK20265 | CUT&Tag Assay Kit (pAG-Tn5) for illumina | 用于染色质免疫沉淀实验，适用于Illumina平台 |
| 建库接头 | RK20290 | Dual DNA Adapter 96 Kit for One-step DNA Lib Prep | 双端CDI，截短型 |
| CUT&Tag 核心酶 | RK20264 | pAG-Tn5 Transposome | 同时具备Tn5转座酶和Protien A/G的功能 |
| RK20550 | pAG-Tn5 Transposase for CUT&Tag | 同时具备Tn5转座酶和Protien A/G的活性功能的新型融合酶 | |
| 配套产品 | AI20021P | 1.5 mL (8-well) Magnetic Separation Device ｛1.5 mL 双排八孔磁力架｝ | 磁力架 |
| AI20022P | 1.5 mL (16-well) Magnetic Separation Device ｛1.5 mL 双排十六孔磁力架｝ | ||
| AI20023P | 1.5 mL (24-well) Magnetic Separation Device ｛1.5 mL 双排二十四孔磁力架｝ | ||
| AI20024P | 0.2 mL (24-well) Magnetic Separation Device Plus｛超强磁力 0.2 mL 双排二十四孔磁力架｝ | ||
| AI20027 | 96-well PCR Plates Magnetic Separation Device ｛96孔板磁力架｝ |
CUT&Tag验证抗体产品列表
| 靶点 | 产品名称 | 物种 | 货号 |
|---|---|---|---|
| Histone H3 | Histone H3 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22348 |
| Acetyl-Histone | Acetyl-Histone H3-K18 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A20735 |
| Acetyl-Histone | Acetyl-Histone H3-K27 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22077 |
| Acetyl-Histone | Acetyl-Histone H3-K56 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22565 |
| Acetyl-Histone | Acetyl-Histone H3-K9 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A21107 |
| Acetyl-Histone | Acetyl-Histone H4-K5 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A23080 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22078 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K9 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A20734 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-K18 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A20680 |
| MonoMethyl-Histone | MonoMethyl-Histone H3-R2 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A19645 |
| DiMethyl-Histone | DiMethyl-Histone H3-K4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22143 |
| TriMethyl-Histone | TriMethyl-Histone H3-K4 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22146 |
| TriMethyl-Histone | TriMethyl-Histone H3-K9 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22295 |
| TriMethyl-Histone | TriMethyl-Histone H3-K27 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22006 |
| TriMethyl-Histone | TriMethyl-Histone H3-K27 Rabbit mAb | Human, Mouse, Rat, Other (Wide Range Predicted) | A22396 |
| c-Myc | [KO Validated] c-Myc Rabbit mAb | Human, Mouse | A19032 |
| CTCF | CTCF Rabbit mAb | Human, Mouse, Rat | A19588 |
| E2F6 | E2F6 Rabbit mAb | Human | A11546 |
| ESE1/ELF3 | ESE1/ELF3 Rabbit mAb | Human, Mouse, Rat | A5236 |
| HMGB2 | HMGB2 Rabbit mAb | Human, Mouse, Rat | A9168 |
| ikaros | Ikaros Rabbit mAb | Human, Mouse | A3565 |
| JunD | JunD Rabbit mAb | Human, Mouse, Rat | A5433 |
| NRF1 | NRF1 Rabbit mAb | Human, Mouse, Rat | A3252 |
| POLR2A | Phospho-POLR2A CTD-S2 Rabbit mAb | Human, Mouse, Rat | AP0996 |
| POLR2A | Phospho-POLR2A-S2/S5 Rabbit mAb | Human, Rat | AP1354 |
| Prdm9 | Prdm9 Rabbit mAb | Human, Mouse, Rat | A20832 |
| Smad4 | [KO Validated] Smad4 Rabbit mAb | Human, Mouse, Rat | A19116 |
| SP1 | SP1 Rabbit mAb | Human, Mouse, Rat | A19649 |
| TEAD1 | TEAD1 Rabbit mAb | Human, Mouse, Rat | A5218 |
| YAP1 | [KO Validated] YAP1 Rabbit mAb | Human, Mouse, Rat | A22650 |
